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KMID : 0545120000100010107
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.107 ~ p.110
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Re-Elicitation with Methyl Jasmonate in Eschscholtzia californica Cell Suspension Cultures
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BYUN SANG-YO
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Abstract
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Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elicitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250㎎/l, which was 2-fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.
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